Characterization of the cervicovaginal microbiota of female beef cattle harboring Campylobacter fetus subsp. venerealis using 16S rDNA gene sequencing.

Bovine genital campylobacteriosis (BGC) is a leading cause of return to estrus in cows. The etiologic agent, Campylobacter fetus subsp. venerealis (Cfv) is transmitted by venereal route. Hence, the surrounding reproductive tract microenvironment could play a role in return to estrus in cows. The presence of Cfv in cervicovaginal mucus of Angus breed females provide three experimental groups, which were subject to bacteriome analyses: 10 Cfv-positive cows (CVP), 10 Cfv-negative cows (CVN), and 10 nonsexually active heifers (NSA). Cows with return to estrus showed higher bacterial richness than NSA. Beta diversity analysis showed a significant difference (P = 0.006) in bacterial composition among the three groups analyzed (CVP, CVN, and NSA). However, no significant difference was found when comparing the CVP versus CVN groups. Ureaplasma and Pseudomonas were the genera most frequently observed in NSA, being Ureaplasma the predictor genus to that group, whereas Alistipes, Bacteroides, Rikenellaceae_RC9_gut_group, UCG-005, and UCG-10 were both significantly more abundant and predictors genera in cows with return to estrus. Our results provide an overview of the cervicovaginal bacterial microbiota in cows harboring Cfv and improve the knowledge of the pathogenesis of BGC.

Molecular survey of hemotropic mycoplasmas in crab-eating raccoons (Procyon cancrivorus) in southern Brazil.

Hemoplasmas are non-cultivable bacterial parasites of erythrocytes that infect domestic and wild animals, as well as humans. Their means of transmission and pathogenesis remain contentious issues and difficult to evaluate in wild animals. Procyon cancrivorus is a South American carnivore and occurs in all Brazilian biomes. In this study, we aimed to investigate occurrences of hemoplasmas infecting P. cancrivorus and to identify their 16S rRNA gene, in southern Brazil. DNA was extracted from spleen and blood samples of P. cancrivorus (n = 9) from different locations. Hemoplasma DNA was detected in six samples, based on 16S rRNA gene amplification and phylogenetic analysis. Four of the six sequences belonged to the "Mycoplasma haemofelis group", which is closely related to genotypes detected in Procyon lotor from the USA; one was within the "Mycoplasma suis group", closely related to "Candidatus Mycoplasma haemominutum"; and one was within the intermediate group between these clusters. Thus, these sequences showed that the molecular identity of hemoplasmas in the population studied was very variable. In five positive animals, Amblyomma aureolatum ticks and a flea (Ctenocephalides felis felis) were collected. The present study describes the first molecular detection of mycoplasmas in P. cancrivorus.

Epidemiological compatibility of Amblyomma sculptum as possible vector and Panthera onca as reservoir of Cytauxzoon spp. in Midwestern Brazil.

Cytauxzoonosis is an acute and highly lethal tick-borne disease of wild and domestic cats, and is widely distributed in Africa, Asia, Europe, the USA and Brazil. So far, only two tick species present on the USA are experimentally confirmed in Cytauxzoon transmission however, in Brazil and other continents, the epidemiology of the disease remains unknown. Evidences points to Panthera onca as a possible reservoir, but there is no evidence to point the vector. Therefore, this study evaluates the presence of Cytauxzoon spp. in wild felids from areas with and without records of Amblyomma sculptum this ixodid for comparison. Overall, 53 blood samples of P. onca, Puma concolor, and Leopardus pardalis from the Midwest region (MR; region with A. sculptum) and 143 blood and/or spleen samples from Leopardus geoffroyi, Leopardus wiedii, Leopardus munoai, Leopardus guttulus, Herpailurus yagouaroundi, L. pardalis, and P. concolor from Rio Grande do Sul State (RS; without A. sculptum). Only one feline sample was negative for Cytauxzoon sp. from MR; no samples from RS were positive. In total, 507 ticks were identified from MR felids, with predominance of A. sculptum (69.23%). In RS, there were 93 ixodids, of which 90.32% were Amblyomma aureolatum. The difference in the tick fauna of the two regions studied (presence/absence of A. sculptum) reflects the results found. This study highlighted A. sculptum as a possible vector since this hemoparasite was abundantly observed in areas where it occurs, also, there was no evidence of Cytauxzoon spp. where it was absent. Additionally, the study supported the suggestion that P. onca is the reservoir for the agent in MR.

Correlation between liver lipidosis, body condition score variation, and hepatic analytes in dairy cows.

Liver lipidosis is a metabolic disorder mostly observed in high yielding dairy cattle, especially during the transition period. The aim of this study was to determine the correlation between hepatic lipid infiltration, biochemical indicators of liver function, and body condition score (BCS) variation in dairy cows. Fifty-one multiparous Holstein cows raised in a confined system were evaluated. Liver biopsies and blood samples were collected, and BCS was measured on days 3 and 28 postpartum. Lipid infiltration was determined by histologic examination. The plasma activity of aspartate aminotransferase, alkaline phosphatase, and gamma-glutamyl transferase and concentration of beta-hydroxybutyrate, non-esterified fatty acids, albumin, total bilirubin, and cholesterol were determined. BCS was measured using objective (camera) and subjective (visual) methods. Mild lipid infiltration was found in 3.92% of cows sampled on day 3 and 5.88% on day 28. Bilirubin was significantly higher on day 3 than on day 28 postpartum, and cholesterol was significantly higher on day 28 than on day 3 in all cows. There was no difference in biochemical analytes between cows with and without lipidosis. On day 3, mean subjective BCS was 3.10 and objective BCS was 3.16, while on day 28, these scores were 2.91 and 2.99, respectively. The calculated liver function index (LFI) was found to be a more sensitive indicator of liver function than the hepatic analytes evaluated. No correlation between BCS variation and lipid infiltration was found. Cholesterol and bilirubin levels showed the most remarkable changes during the early postpartum period. LFI is a potential indicator of postpartum liver function.

A lipidose hepática é um distúrbio metabólico principalmente observado nos bovinos de leite de alto rendimento, especialmente no período de transição. O objetivo deste estudo foi determinar a correlação entre infiltração lipídica hepática, indicadores bioquímicos de função hepática e variação da condição corporal em bovinos leiteiros. Foram avaliadas cinquenta e um vacas multíparas de raça Holandesa em confinamento. Coletaram-se biopsias de fígado e amostras de sangue, e a condição corporal (BCS) aferiu-se nos dias 3 e 28 pós-parto. A infiltração lipídica determinou-se mediante avaliação histológica. Mensuraram-se a atividade da aspartato aminotransferase, fosfatase alcalina e da gama-glutamil transferase, concentração de beta-hidroxibutirato, ácidos graxos não esterificados, albumina, bilirrubina total e colesterol. A BCS mediu-se utilizando método objetivo (câmera) e subjetivo (visualmente). Observou-se discreta infiltração lipídica em 3,92% dos bovinos amostrados no dia 3 e 5,88% no dia 28. Em todos os bovinos a bilirrubina foi significativamente mais alta no dia 3 do que no dia 28 e o colesterol foi superior no dia 28 do que no dia 3. Não houve diferença nos analitos bioquímicos dos bovinos com e sem lipidose. No dia 3, a média subjetiva da BCS foi 3,10 e a objetiva 3,16, enquanto no dia 28, obtiveram-se valores de 2,91, e 2,99 respectivamente. O índice calculado de função hepática mostrou ser um indicador mais sensível da função hepática do que os analitos avaliados individualmente. Não houve correlação entre a variação do BCS e infiltração lipídica.

Low-Intensity, High-Frequency Grazing Positively Affects Defoliating Behavior, Nutrient Intake and Blood Indicators of Nutrition and Stress in Sheep.

The intensity and frequency of grazing affect the defoliating strategy of ruminants, their daily nutrient intake, thus nutrition and physiological status. Italian ryegrass (Lolium multiflorum Lam.) pastures were grazed by sheep either under a low-intensity/high-frequency grazing strategy (Rotatinuous stocking; RN) with nominal pre- and post-grazing sward heights of 18 and 11 cm, respectively, or under a high-intensity/low-frequency strategy (traditional rotational stocking; RT) with nominal pre- and post-grazing sward heights of 25 and 5 cm, respectively. Treatments were arranged under a complete randomized design and evaluated over two periods, in different years. In 2017, the aim was to depict the type of bites that sheep perform during the grazing-down and associate them to the grazing management strategy according to their relative contribution to the diet ingested. In 2018 we estimated the total nutrient intake and evaluated blood indicators of the nutritional status and immune response to stress of sheep. The bite types accounting the most for the diet ingested by RN sheep were those performed on the "top stratum" of plants with around 20, 15, and 25 cm, whereas the type of bites accounting the most for the diet of RT sheep were those performed on "grazed plants" with around 10, 5, and ≤ 3 cm. In 2018, the RN sheep increased by 18% the total organic matter (OM) intake and by 20-25% the intake of soluble nutrients (i.e., crude protein, total soluble sugars, crude fat), digestible OM and of metabolizable energy, and had 17.5, 18, and 6.1% greater blood concentration of glucose, urea nitrogen (BUN) and albumin, respectively, but 17% lower blood neutrophil-to-lymphocyte (N:L) ratio. Sheep grazing vegetative Italian ryegrass pastures under the low-intensity/high-frequency grazing strategy (RN) ingested a diet of better quality from bites allocated on the top stratum of plants, had greater intake of soluble nutrients and blood parameters positively associated with nutritional status and immune response to stress.

Clinical and microbiological characterization of subclinical bacteriuria and sporadic bacterial cystitis in dogs with spontaneous hypercortisolism.

Study's aims were to characterize subclinical bacteriuria (SB) and sporadic bacterial cystitis (SBC) in dogs with spontaneous hypercortisolism (HC). Prospective cross-sectional design divided patients as newly diagnosed (n = 27), poorly controlled (n  = 21), well controlled (n  = 34), and controls (n  = 19). Urine culture positive results were identified by MALDI-TOF and submitted to antibiogram. Escherichia coli was the most common microorganism (36%). The majority of positive cultures in HC were SB (12.2%). All 4.1% SBC cases were in well controlled HC cases. Bacteriuria correlated with low urine specific gravity and low lymphocyte count. HC degree of control correlated with leukocyturia. SB/SBC cases were treated based in antimicrobial susceptibility leading to microbiological cure in 75% of HC cases. Persistent infections occurred only in SB cases, all by E. coli which became more resistant. SB/SBC prevalence in canine HC is actually lower. Further evidence for current ISCAID guideline contraindication for SB treatment due to HC were provided.

First molecular detection of Mycoplasma ovis (Hemotropic mycoplasmas) from Sheep in Brazil / Primeira detecção molecular de Mycoplasma ovis (Micoplasmas Hemotrópicos) em Ovinos no Brasil

Abstract Mycoplasma ovis is an emerging zoonotic pathogen with a worldwide distribution and can cause mild to severe hemolytic anemia, icterus, and poor weight gain in animals. Although M. ovis has been described in small ruminants worldwide, data on M. ovis in sheep in Brazil is unknown. The objective of the present study was to present the first report of hemotropic mycoplasma (HM) in sheep from Brazil. We evaluated factors associated with this infection, such age group, tick presence, and anemia. Blood samples were collected from 33 sheep from a farm in southern Brazil and screened for hemoplasmas using PCR. Out of 33 samples, 26 (78.8%) tested positive for M. ovis. The sequencing of positive samples showed 100% identity with multiple M. ovis 16S rDNA sequences. No association was observed between the presence of M. ovis and the FAMACHA© score (p = 0.620). Rhipicephalus (Boophilus) microplus (15/33, 45.4%) was the tick species found on the animals. No significant association between M. ovis infection and presence of ticks (p = 0.4134) and age group (p = 0.4221) was observed. This is the first report of M. ovis infection in sheep from Brazil and only the second report of this pathogen in sheep in Latin America.

Resumo Mycoplasma ovis é um patógeno zoonótico emergente com distribuição mundial e pode causar anemia hemolítica de leve a grave, icterícia e baixo ganho de peso em animais. Embora M. ovis tenha sido descrito em pequenos ruminantes em todo o mundo, os dados sobre M. ovis em ovinos no Brasil são desconhecidos. O objetivo deste estudo foi apresentar o primeiro relato de micoplasmas hemotrópicos em ovinos no Brasil. Avaliamos os fatores associados a essa infecção, como faixa etária, presença de carrapatos e anemia. Amostras de sangue foram coletadas de 33 ovelhas de uma fazenda no sul do Brasil e testadas para hemoplasmas usando a PCR. Das 33 amostras, 26 (78,8%) apresentaram resultado positivo. O sequenciamento das amostras positivas mostrou 100% de identidade com múltiplas sequências de M. ovis 16S rDNA. Não foi observada associação entre a presença de M. ovis e o escore FAMACHA© (p = 0,620). Rhipicephalus (Boophilus) microplus (15/33, 45,4%) foi a espécie de carrapato encontrada nos animais. Não houve associação significativa entre infecção por M. ovis e presença de carrapatos (p = 0,4134) e faixa etária (p = 0,4221). Este é o primeiro relato de infecção por M. ovis em ovinos no Brasil e o segundo relato deste patógeno em ovinos na América Latina.

First molecular detection of Mycoplasma ovis (Hemotropic mycoplasmas) from Sheep in Brazil.

Mycoplasma ovis is an emerging zoonotic pathogen with a worldwide distribution and can cause mild to severe hemolytic anemia, icterus, and poor weight gain in animals. Although M. ovis has been described in small ruminants worldwide, data on M. ovis in sheep in Brazil is unknown. The objective of the present study was to present the first report of hemotropic mycoplasma (HM) in sheep from Brazil. We evaluated factors associated with this infection, such age group, tick presence, and anemia. Blood samples were collected from 33 sheep from a farm in southern Brazil and screened for hemoplasmas using PCR. Out of 33 samples, 26 (78.8%) tested positive for M. ovis. The sequencing of positive samples showed 100% identity with multiple M. ovis 16S rDNA sequences. No association was observed between the presence of M. ovis and the FAMACHA© score (p = 0.620). Rhipicephalus (Boophilus) microplus (15/33, 45.4%) was the tick species found on the animals. No significant association between M. ovis infection and presence of ticks (p = 0.4134) and age group (p = 0.4221) was observed. This is the first report of M. ovis infection in sheep from Brazil and only the second report of this pathogen in sheep in Latin America.

Changes in arterial partial pressure and end-expired pressure of carbon dioxide in prepubescent and adult bitches undergoing ovariohysterectomy by videolaparoscopy or conventional laparotomy / Variações nas pressões parciais arteriais e expiradas de dióxido de carbono em cadelas pré-púberes e adultas submetidasà ovário histerectomiavídeo laparoscópica ou convencional

ABSTRACT: This paper aimed to determine arterial partial pressure of carbon dioxide (PaCO2), end-expired CO2 pressure (ETCO2), and the difference between arterial and end-expired CO2 pressure (Pa - ETCO2) in prepubescent and adult bitches undergoing videolaparoscopic or conventional ovariohyterectomy (OH). Forty bitches were randomly assigned to four groups: Conventional Adult (CA), Conventional Pediatric (CP), Videolaparoscopic Adult (VA) and Videolaparoscopic Pediatric (VP). Pulse rate (PR), respiratory rate (RR), systolic, mean, and diastolic arterial pressures (SAP, MAP, DAP), ETCO2, peak inspiratory pressure (PIP), pH, arterial partial pressure of oxygen (PaO2), PaCO2, base excess (BE) and HCO3 - were measured. Based on the PaCO2 and ETCO2 values, Pa-ETCO2 was determined. There was no significant difference in PaCO2 between the VA (42.5±5.2 to 53.7±5.2) and VP (48.4±5.4 to55.4±5.7) groups. During the postoperative period, all groups presented with hypertension. However, mild hypertension (SAP 150 to 159mmHg) was observed in the VP group as compared to severe hypertension (SAP>180mmHg) in the CA group, suggesting that both the age range and videolaparoscopic OH are associated with lower levels of hypertension during the postoperative period in dogs.

RESUMO: O objetivo deste estudo foi determinar a pressão parcial de dióxido de carbono (PaCO2), pressão ao final da expiração de CO2 (ETCO2) e diferença artério-alveolar de CO2 (Pa-ETCO2) em cadelas pré-púberes e adultas submetidas à ovário-histerectomia (OH) videolaparoscópica ou convencional. Foram distribuídas 40 cadelas em quatro grupos: Convencional Adulto (CA), Convencional Pediátrico (CP), Videolaparoscópico Adulto (VA) e Videolaparoscópico Pediátrico (VP). Foram mensurados frequência de pulso (FP), frequência respiratória (FR), pressões arteriais sistólica (PAS), média (PAM) e diastólica (PAD), ETCO2, pressão de pico inspiratória (PIP), pH, pressão parcial arterial de oxigênio (PaO2), PaCO2, excesso de bases (EB) e HCO3 -. Com base nos valores de PaCO2 e ETCO2 encontrados, foi determinada a Pa-ETCO2. Não foram encontradas diferenças significativas nos valores de PaCO2 entre os grupos VA (42.5±5.2-53,7±5,2) e VP (48.4±5.4 - 55,4±5,7). Todos os grupos apresentaram hipertensão arterial no período pós-operatório. Entretanto, o grupo VP apresentou hipertensão moderada (PAS 150-159mmHg) em comparação ao grupo CA, que apresentou hipertensão severa (PAS>180 mmHg), sugerindo que tanto a faixa etária, quanto a execução de OH por videolaparoscopia, estão associadas a menores taxas de hipertensão pós-operatória em cadelas.

Rangeliosis: histopathological analysis, hematology and molecular detection of canine Rangelia vitalii in Rio Grande do Sul, Brazil / Rangeliose: achados anatomopatológicos, hematológicos e detecção molecular de Rangelia vitalii em cães no Rio Grande do Sul, Brasil

ABSTRACT: In the period from January 2004 to December 2015, 56 dogs were diagnosed with rangeliosis in the Setor de Patologia Veterinária at Universidade Federal do Rio Grande do Sul (SPV-UFRGS). The main hematological abnormalities were thrombocytopenia and anemia. The affected dogs showed signs of apathy, anorexia, fetid and bloody diarrhea, vomiting, and dehydration. At necropsy, the main changes were jaundice, splenomegaly, hepatomegaly, and lymphadenomegaly. Histological analyses revealed parasitophorous vacuoles of Rangelia vitalii in cytoplasmic endothelial cells, mainly in the heart, kidneys, lymph nodes, intestines, and pancreas. Inflammation characterized by mononuclear cells was predominant in the analysis, and most was due to the presence of plasma cells. Other lesion types observed were lymphoid hyperplasia, extramedullary hematopoiesis, erythrophagocytosis, and erythroid lineage hyperplasia in bone marrow. Of the total number of animals, 49 were diagnosed using necropsy and histological analysis, and seven were diagnosed using a molecular analysis (i.e., PCR and genetic sequencing of blood samples). This paper presented a different method of diagnosing rangeliosis in canines. This approach involved histological methods including the quantification and determination of the intensity and distribution of the infectious agent in different organs.

RESUMO: No período de janeiro de 2004 a dezembro de 2015, 56 caninos domésticos obtiveram o diagnóstico de rangeliose no Setor de Patologia Veterinária da Universidade Federal do Rio Grande do Sul (UFRGS). Os cães apresentaram sinais de apatia, anorexia, diarreia fétida e sanguinolenta, êmese e desidratação. As principais alterações hematológicas foram trombocitopenia e anemia. Na necropsia as principais alterações foram icterícia, esplenomegalia, hepatomegalia e linfadenomegalia. Na análise histológica observou-se vacúolos parasitóforos de Rangelia vitalii no citoplasma de células endoteliais, principalmente no coração, rins, linfonodos, intestinos e pâncreas. A inflamação mononuclear foi predominante na análise, sendo que a maioria deu-se pela presença de plasmócitos. Entre outras lesões frequentemente observadas estão hiperplasia linfoide, hematopoiese extramedular e eritrofagocitose, e hiperplasia de linhagem eritroide em medula óssea. Do total, 49 cães foram diagnosticados através de necropsia e análise histológica, e 7 animais através de análise molecular da PCR e sequenciamento genético de amostras de sangue. Este trabalho apresenta um diagnóstico diferencial de rangeliose em caninos, através do método histológico de quantificação e determinação de intensidade e distribuição do agente em diferentes órgãos.

Identification, occurrence and clinical findings of canine hemoplasmas in southern Brazil.

Hemoplasmas are ubiquitous pleomorphic and epicellular bacteria detected in erythrocytes in several species. In Brazil, studies on hemoplasmas have not included information on occurrence, clinical signs, and risk factors in dogs. This paper investigates the occurrence of hemoplasmas in dogs, focusing on risk factors and clinical status. Conventional PCR for the four types of canine hemoplasmas was performed in 331 blood samples collected from dogs clinically treated at a teaching veterinary hospital. Of all samples, 17/331 (5.1%) were positive for Mycoplasma haemocanis and 6/331 (1.8%) were positive for a 'Candidatus Mycoplasma haemominutum-like' organism. Risk factors included the presence of vectors, old age, dog bite wounds, and neoplastic diseases. In the multivariate analysis, a 4.40 odds ratio in dogs with vector-borne diseases indicated risk for hemoplasmosis. There was correlation between hemoplasma infection and neoplastic disease, suggesting that neoplastic conditions are a risk factor for hemoplasma infection in dogs.

Parâmetros de bioquímica sérica de machos, fêmeas e filhotes de Araras canindé (Ara ararauna) saudáveis mantidas em cativeiro comercial / Serum biochemical parameters of healthy male, female and young blue-and-yellow macaws (Ara ararauna) bred in captivity

O Brasil possui um considerável número de espécies de psitacídeos catalogados, perfazendo cerca de 80 espécies, sendo que as Araras canindé (Ara ararauna), uma das maiores representantes dessa ordem, podem ser encontradas em florestas nas diversas regiões brasileiras. O Instituto Brasileiro do Meio Ambiente e dos Recursos Naturais Renováveis (IBAMA) normalizou a comercialização de animais da fauna silvestre provenientes de criadouros e conseqüentemente ocorreu um aumento do número destes animais como de estimação. Atualmente, há pouco conhecimento sobre os parâmetros clínicos e laboratoriais de espécies silvestres em cativeiro. O presente trabalho teve como objetivo determinar parâmetros de bioquímica sangüínea de Araras canindé (Ara ararauna) saudáveis de sexo e faixa etária distintas mantidas em um criatório comercial com alimentação e manejo controlados e padronizados. Foram colhidas amostras de sangue de 35 araras canindé (11 filhotes e 24 adultos) e remetidas ao Laboratório de Análises Clínicas Veterinárias da Universidade de Passo Fundo (UPF) para determinação dos seguintes indicadores bioquímicos: ácido úrico (AU), albumina (ALB), aspartato aminotransferase (AST), cálcio (Ca), colesterol (Col), creatina quinase (CK), fosfatase alcalina (FA), fósforo inorgânico (Pi), frutosamina (Fru), gama glutamil transferase (GGT), proteínas totais (PT) e uréia (UR). Entre as aves adultas, foram encontradas diferenças significativas nos valores de CK (superior nos machos), Ca e AU (superiores nas fêmeas). Entre aves adultas e filhotes foram constatadas diferenças significativas nos valores de AU, Ca, ALB, COL, FA, Pi e FRU. A maioria das alterações detectadas pode ser relacionada à diferença na dieta fornecida e à condição fisiológica de cada categoria de aves. Os dados obtidos podem ser utilizados como parâmetros de referência para as araras canindé brasileiras.

Several psittacines have been cataloged in Brazil, totaling nearly 80 species. The blue-and-yellow macaw (Ara ararauna), the most representative species of the Psittaciformes order, lives in the forest areas of different Brazilian regions. IBAMA, Brazilian environmental protection agency, has authorized the trade of wild animals raised in breeding facilities; consequently, their use as household pets has increased ever since. Currently, too little is known about clinical and laboratory parameters of wild species bred in captivity. The aim of the present study was to determine the serum biochemical parameters of healthy blue-and-yellow macaws (Ara ararauna) of different sexes and ages reared in a commercial breeding facility under controlled and standardized feeding and management practices. Blood samples were collected from 35 blue-and-yellow macaws (11 fledglings and 24 adults) and sent to the Laboratory of Veterinary Clinical Pathology of Universidade de Passo Fundo (UPF) for measurement of the following biochemical parameters: uric acid (UA), albumin (Alb), aspartate aminotransferase (AST), calcium (Ca), cholesterol (Chol), creatine kinase (CK), alkaline phosphatase (AP), inorganic phosphorus (iP), fructosamine (Fru), gamma-glutamyl transferase (GGT), total protein (TP) and urea nitrogen (Ur). There were significant differences among adult macaws with regard to CK levels (higher in males) and to Ca and UA levels (higher in females). Differences were noted among adults and fledglings with respect to UA, Ca, Alb, Chol, AP, iP and Fru. Most of the findings can be ascribed to the different feeding regimens and ages of the birds analyzed. The data obtained herein can be used as reference parameters for Brazilian blue-and-yellow macaws.

Lavado traqueobrônquico auxiliado por endoscópio rígido ou por tubo endotraqueal em cães / Tracheobronchic washing in dogs by means of rigid endoscopy or endotracheal tube

Com o objetivo de se avaliarem os resultados do uso de endoscopia rígida e da técnica por tubo endotraqueal de colheita de líquido traqueobrônquico, foram utilizados 28 cães, errantes ou domiciliados, distribuídos em dois grupos. Nos cães do grupo 1, aplicou-se a técnica de lavado traqueobrônquico em 14 cães, sendo sete sadios e sete com sinais clínicos respiratórios. Nos do grupo 2, utilizou-se a mesma técnica, entretanto com endoscopia rígida, em sete cães sadios e em sete cães com sinais clínicos de doença respiratória. Os dados evidenciaram que o procedimento utilizando-se endoscópio rígido resultou em menor volume de solução infundida e em maior porcentagem de solução recuperada. Não houve diferença significativa quanto ao tempo de duração da execução da técnica. A contagem bacteriana foi mais alta nas amostras coletadas por endoscópio rígido, porém não houve diferença significativa na contagem de células nucleadas totais. Portanto, o uso da endoscopia rígida para colheita de líquido traqueobrônquico mostrou-se mais vantajosa do que a técnica convencional utilizando-se o tubo endotraqueal, pois fornece imagens das vias aéreas e permite acompanhamento visual da lavagem.

The present study aimed to evaluate the results of rigid endoscopy and endotracheal tube techniques for collecting tracheobronchic fluid from dogs. Twenty eight erratic or housed dogs, were allocated into to two groups. In group 1, tracheobronchic washing was applied to seven healthy dogs and seven dogs with clinical signs of respiratory disease. In group 2, seven healthy and seven dogs with respiratory disease were submitted to endo-bronchial washing by rigid endoscopy. Using rigid endoscopy, a lower volume was introduced but a higher percentage of washing fluid was recovered. There was no difference regarding the time necessary to perform both techniques. Bacterial counts were higher in samples collected by rigid endoscopy, but there was no difference in total nucleated cell counts from these samples. Thus, the use of rigid endoscopy to collect tracheobronchic fluid was found to be advantageous when compared to the conventional technique using endotracheal tube.

Análise comparativa entre microbiologia convencional, ELISA e PCR para detecção de Salmonella enteritidis, S. typhimurium, S. gallinarum e S. pullorum em carne de frango contaminada artificialmente / Comparative analysis between conventional microbiological method, ELISA and PCR for the detection of Salmonella enteritidis, S. typhimurim, S. gallinarum and S. pullorum in artificially contaminated broiler meat

O Presente trabalho teve como objetivo realizar uma análise comparativa das técnicas de Reação em Cadeia pela Polimerase (PCR) e Ensaioo Imunoenzimático (ELlSA - SALVIA) com o método microbiológico convencional para detecção e Salmonella Enteridis(SE), S. typhimurium (ST), S. gallinarum (SG) e S. pullorum (SP) em carne de frango. As amostras foram contaminadas artificialmente com diluições de 10-7, 10-8 e 10-9 para SE e ST e de 10-4, 10-5 e 10-6 para SG e SP, com cinco repetições de cada diluição totalizando 300 análises. Os testes foram realizados em cinco diferentes laboratórios para a validação das técnicas. Na avaliação geral dos dados obtidos, a microbiologia convencional obteve 56,67% (170/300) de recuperação das amostras contaminadas artificialmente, enquanto as técnicas de ELlSA e PCR representaram 71% (213/300) e 75% (225/300), respectivamente. A análise dos resultados de detecção de Salmonella através dos testes ELlSA e PCR, em relação ao microbiológico convencional, apresentaram diferença estatística (p=0,0001, teste de MacNemar). Não houve diferença significativa entre os resultados da PCR e do ELlSA. Os resultados alcançados demonstraram que, comparado ao microbiológico convncional, tanto o ELlSA quanto a PCR foram eficazes para detecção dos sorovares de Salmonella nas amostras de carne de frango avaliadas

Ocorrência de salmonella em abatedouros de aves com tecnologia totalmente automatizada (grande porte), semi automatizada (médio porte) e semi automatizada (pequeno porte) / Occurrence of salmonella in poultry slaughterlouses with technology grossly automated (big carriage), is automated (medium carriage) and is automated (small carriage)

Foi investigada a presença de Salmonella em diferentes etapas do processamento industrial de três categorias de abatedouros avícolas, sendo um com tecnologia totalmente automatizada um com tecnologia semi-automatizada de pequeno porte. Foram coletados 60 swabs cloacais antes das aves passarem pela depenadeira, 60 carcaças após a evisceração e antes da passagem pelo chiller e 60 carcaças após a passagem pelo chiller, totalizando 180 amostras.No abatedouro com tecnologia totalmente automatizada foram identificadas 30 (50 por cento) amostras positivas para salmonela, sendo 12/20 (60 por cento) no swab cloacal, 14/20 (70 por cento) antes do chiller e 4/20 (20 por cento) após o chiller. No abatedouro com tecnologia semi-automatizada, 4/20 (20 por cento) amostras foram positivas no swab cloacal, 5/20 (25 por cento) antes do chiller e 8/20 (40 por cento) após o chiller, totalizando 17/60 (28,3 por cento) amostras positivas, enquanto que no abatedouro com tecnologia manual não houve isolamento de Salmonella. Das 180 amostras analisadas, isolou-se Salmonella em 47 (26,11 por cento), das quais 63,9 por cento (30/47) foram S.Heidelberg, 31,9 por cento (15/47) S.Enteritidis, 2,1 por cento (1/47) S. Worthington e 2,1 por cento (1/47) S. Tennessee. Os dados observados na presente pesquisa permitem concluir que:- a detecção de 26,7 por cento de lotes positivos para Salmonella indica um alto grau de contaminação das aves quando da chegada no abatedouro;-a automatização da evisceração de carcaças de frangos aumenta a contaminação por salmonelas;-o sistema de pré-resfriamento de carcaças de frango, quando bem conduzido, diminui a contaminação, ao tempo que pode ampliá-la quando executado de forma errônea;- todos os sorovares detectados foram salmonelas paratíficas, demonstrando relevância para saúde pública;- os critérios de julgamento macroscópico adotados e preconizados pela legislação são insuficientes para o diagnóstico de Salmonella, devendo ser complementados por análises microbiológicas.

Mineral deficiencies in beef cattle from southern Brazil / Deficiências minerais em gado de corte no sul do Brasil

Minerals play an important role in the cattle metabolism with great impact in productive and reproductive performance of animals raised on extensive grasslands. At present, there is a lack of information concerning mineral deficiency throughout the analysis of biological fluids in Rio Grande do Sul State (southern Brazil). The Central Valley region of this State is characterized by extensive production on natural pastures with poor mineral supplementation. The low levels of minerals found by previous studies on pastures in this area motivated this research. The aim of the present work was to detect possible mineral deficiencies in beef cows by determining concentrations of minerals in biological fluids and in pasture. There were determined concentrations of phosphorus, calcium, copper, zinc, glutathion peroxidase (as indicator of selenium status) and thyroxine (as indicator of iodine status) in blood, sodium and potassium in saliva, and calcium, phosphorus, potassium, zinc, copper, sodium and iron in pastures, in four different periods of the reproductive cycle as follows: service period (artificial insemination), clean up bulls period, end of gestation and early lactation. Four herds were selected in Cachoeira do Sul County for this study which included 112 animals. The results showed marginal deficiencies of phosphorus, copper, iodine and selenium. Calcium concentration was below normal level and could be related to the low protein content of pasture. Most critical periods were the end of gestation and the early lactation.

Os minerais são de grande importância no metabolismo dos bovinos, tendo impacto no desempenho produtivo e reprodutivo dos animais. Atualmente há poucos relatos no Rio Grande do Sul com relação ao diagnóstico de deficiências minerais mediante a análise de fluidos biológicos em animais a campo. A região da Depressão Central do Rio Grande do Sul é caracterizada pela produção extensiva de gado de corte em campo nativo com manejo precário da suplementação mineral, apresentando baixos níveis de alguns minerais nas pastagens. O objetivo do presente trabalho foi diagnosticar possíveis deficiências minerais em vacas de corte na região da Depressão Central mediante a dosagem de indicadores minerais em fluidos biológicos e na pastagem. Foram determinadas concentrações de fósforo, cálcio, cobre, zinco, glutation peroxidase (como indicador de selênio) e tiroxina (como indicador de iodo) no sangue, sódio e potássio na saliva e sódio, e cálcio, fósforo, potássio, zinco, cobre, sódio e ferro na pastagem. Foram estudadas quatro propriedades em Cachoeira do Sul, em quatro momentos do ciclo reprodutivo: período de serviço (inseminação artificial), período de repasse de touros, final da gestação e início da lactação, num total de 112 animais. O perfil mineral indicou deficiência marginal de fósforo, cobre, iodo e selênio. O cálcio sérico apresentou-se diminuído, possivelmente relacionado com o baixo conteúdo de proteína na pastagem. Os períodos mais afetados foram o final da gestação e o início da lactação indicando que essas categorias de produção têm maior exigências metabólicas.